A subcloning strategy for DNA sequence analysis
نویسندگان
چکیده
منابع مشابه
A subcloning strategy for DNA sequence analysis.
We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced dir...
متن کاملDNA Sequence Fragment Containing C to A Mutation as a Convenient Mutation Standard for DHPLC Analysis
Objective(s): Denaturing high performance liquid chromatography (DHPLC) is a high throughput approach for screening DNA sequence variations. To assess oven calibration, cartridge performance, buffer composition and stability, the WAVE Low and High Range Mutation Standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. The purpose of this study was to provi...
متن کاملSubcloning of the Histone DNA Sequences
The purification and characterization of histone mRNA is a relatively straightforward procedure if conducted with the RNA from early cleavage stage sea urchin embryos [1 -6 ] where histone mRNA is the most abundant mRNA component found in small polysomes [1, 2]. Consequently this has led to an extensive characterization of the different subtypes of histone mRNA [4 -9 ] as well as to their use a...
متن کاملdna sequence fragment containing c to a mutation as a convenient mutation standard for dhplc analysis
objective(s): denaturing high performance liquid chromatography (dhplc) is a high throughput approach for screening dna sequence variations. to assess oven calibration, cartridge performance, buffer composition and stability, the wave low and high range mutation standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. the purpose of this study was to provi...
متن کاملA computer package for DNA sequence analysis
We have developed a comprehensive software-hardware package for a CP/M based or other microcomputer which can perform four major functions in a molecular biology laboratory: (1) the acquisition and analysis of DNA sequence data, (2) word processing in the preparation of scientific manuscripts and figures, (3) general lab computation and data reduction, and (4) as a data terminal for communicati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.23.5541